21-plex DiLeu Isobaric Tags for High-Throughput Quantitative Proteomics.

ABSTRACT: Isobaric tags enable multiplexed quantitative analysis of many biological samples in a single LC-MS/MS experiment. As a cost-effective alternative to expensive commercial isobaric tagging reagents, we developed our own custom N,N-dimethylleucine "DiLeu" isobaric tags for quantitative proteomics. Here, we present a new generation of DiLeu tags that achieves 21-plex quantification in high-resolution HCD MS/MS spectra via distinct reporter ions that differ in mass from each other by a minimum of 3 mDa. The 21-plex set retains the compact tag structure and existing isotopologues of the 12-plex set but includes nine new reporter variants formulated with unique configurations of ¹³C, ¹⁵N, and ²H stable isotopes, each synthesized in-house via a stepw

SUBMITTER: Frost DC 

PROVIDER: S-EPMC7306224 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature