Variations of Elastic Modulus and Cell Volume with Temperature for Cortical Neurons.

ABSTRACT: Neurons change their growth dynamics and mechanical properties in response to external stimuli such as stiffness of the local microenvironment, ambient temperature, and biochemical or geometrical guidance cues. Here we use combined atomic force microscopy (AFM) and fluorescence microscopy experiments to investigate the relationship between external temperature, soma volume, and elastic modulus for cortical neurons. We measure how changes in ambient temperature affect the volume and the mechanical properties of neuronal cells at both the bulk (elastic modulus) and local (elasticity maps) levels. The experimental data demonstrate that both the volume and the elastic modulus of the neuron soma vary with changes in temperature. Our results show a decrease by a factor of 2 in the soma elastic m

SUBMITTER: Sunnerberg JP 

PROVIDER: S-EPMC7306228 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature