Dynamic competition for hexon binding between core protein VII and lytic protein VI promotes adenovirus maturation and entry.

ABSTRACT: Adenovirus minor coat protein VI contains a membrane-disrupting peptide that is inactive when VI is bound to hexon trimers. Protein VI must be released during entry to ensure endosome escape. Hexon:VI stoichiometry has been uncertain, and only fragments of VI have been identified in the virion structure. Recent findings suggest an unexpected relationship between VI and the major core protein, VII. According to the high-resolution structure of the mature virion, VI and VII may compete for the same binding site in hexon; and noninfectious human adenovirus type 5 particles assembled in the absence of VII (Ad5-VII-) are deficient in proteolytic maturation of protein VI and endosome escape. Here we show that Ad5-VII- particles are trapped in the endosome because they fail to increase VI exposur

SUBMITTER: Hernando-Perez M 

PROVIDER: S-EPMC7306765 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature