ASY1 acts as a dosage-dependent antagonist of telomere-led recombination and mediates crossover interference in <i>Arabidopsis</i>.

ABSTRACT: During meiosis, interhomolog recombination produces crossovers and noncrossovers to create genetic diversity. Meiotic recombination frequency varies at multiple scales, with high subtelomeric recombination and suppressed centromeric recombination typical in many eukaryotes. During recombination, sister chromatids are tethered as loops to a polymerized chromosome axis, which, in plants, includes the ASY1 HORMA domain protein and REC8-cohesin complexes. Using chromatin immunoprecipitation, we show an ascending telomere-to-centromere gradient of ASY1 enrichment, which correlates strongly with REC8-cohesin ChIP-seq data. We mapped crossovers genome-wide in the absence of ASY1 and observe that telomere-led recombination becomes dominant. Surprisingly, asy1/+ heterozygotes also remodel cr

SUBMITTER: Lambing C 

PROVIDER: S-EPMC7306779 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature