Force and phosphate release from Arp2/3 complex promote dissociation of actin filament branches.

ABSTRACT: Networks of branched actin filaments formed by Arp2/3 complex generate and experience mechanical forces during essential cellular functions, including cell motility and endocytosis. External forces regulate the assembly and architecture of branched actin networks both in vitro and in cells. Considerably less is known about how mechanical forces influence the disassembly of actin filament networks, specifically, the dissociation of branches. We used microfluidics to apply force to branches formed from purified muscle actin and fission yeast Arp2/3 complex and observed debranching events in real time with total internal reflection fluorescence microscopy. Low forces in the range of 0 pN to 2 pN on branches accelerated their dissociation from mother filaments more than two orders of magnitude

SUBMITTER: Pandit NG 

PROVIDER: S-EPMC7306818 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature