The interactome of the prostate-specific protein Anoctamin 7.

ABSTRACT:

Background

Elevated Anoctamin 7 (ANO7) expression is associated with poor survival in prostate cancer patients.

Objective

The aim was to discover proteins that interact with ANO7 to understand its functions and regulatory mechanisms.

Methods

The proximity-dependent biotin identification (BioID) method was utilized. ANO7 fused to biotin ligase was transiently transfected into LNCaP cells, and the biotinylated proteins were collected and analysed by mass spectrometry. Four identified proteins were stained with dual fluorescent immunostaining and visualized using Stimulated emission depletion microscopy (STED).

Results

After bioinformatic filtering steps, 64 potentially ANO7-interacting proteins were identified and analysed with the GO enrichment analysis tool. On

SUBMITTER: Kaikkonen E 

PROVIDER: S-EPMC7306890 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature