The human mitochondrial 12S rRNA m⁴C methyltransferase METTL15 is required for mitochondrial function.

ABSTRACT: Mitochondrial DNA gene expression is coordinately regulated both pre- and post-transcriptionally, and its perturbation can lead to human pathologies. Mitochondrial rRNAs (mt-rRNAs) undergo a series of nucleotide modifications after release from polycistronic mitochondrial RNA precursors, which is essential for mitochondrial ribosomal biogenesis. Cytosine N⁴-methylation (m⁴C) at position 839 (m⁴C839) of the 12S small subunit mt-rRNA was identified decades ago; however, its biogenesis and function have not been elucidated in detail. Here, using several approaches, including immunofluorescence, RNA immunoprecipitation and methylation assays, and bisulfite mapping, we demonstrate that human methyltransferase-like 15 (METTL15), encoded by a nuclear gene,

SUBMITTER: Chen H 

PROVIDER: S-EPMC7307190 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature