Removal of Fc Glycans from [⁸⁹Zr]Zr-DFO-Anti-CD8 Prevents Peripheral Depletion of CD8⁺ T Cells.

ABSTRACT: The N-linked biantennary glycans on the heavy chain of immunoglobulin G (IgG) antibodies (mAbs) are instrumental in the recognition of the Fc region by Fc-gamma receptors (FcγR). In the case of full-length mAb-based imaging tracers targeting immune cell populations, these Fc:FcγR interactions can potentially deplete effector cells responsible for tumor clearance. To bypass this problem, we hypothesize that the enzymatic removal of the Fc glycans will disrupt Fc:FcγR interactions and spare tracer-targeted immune cells from depletion during immunopositron emission tomography (immunoPET) imaging. Herein, we compared the in vitro and in vivo properties of ⁸⁹Zr-radiolabeled CD8-specific murine mAb (anti-CD8_wt, clone 2.43), a well-known depleting mAb, a

SUBMITTER: White JM 

PROVIDER: S-EPMC7307332 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature