Targeting the coding sequence: opposing roles in regulating classical and non-classical MHC class I molecules by miR-16 and miR-744.

ABSTRACT:

Background

To control gene expression, microRNAs (miRNAs) are of key importance and their deregulation is associated with the development and progression of various cancer types. In this context, a discordant messenger RNA/protein expression pointing to extensive post-transcriptional regulation of major histocompatibility complex (MHC) class I molecules was already shown. However, only a very limited number of miRNAs targeting these molecules have yet been identified. Despite an increasing evidence of coding sequence (CDS)-located miRNA binding sites, there exists so far, no detailed study of the interaction of miRNAs with the CDS of MHC class I molecules.

Methods

Using an MS2-tethering approach in combination with small RNA sequencing, a number of putative miRNAs binding to

SUBMITTER: Friedrich M 

PROVIDER: S-EPMC7307530 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature