DAXX mediates high phosphate-induced endothelial cell apoptosis in vitro through activating ERK signaling.

ABSTRACT:

Backgroud and purpose

Hyperphosphatemia, which is a high inorganic phosphate (Pi) level in the serum, promotes endothelial cells dysfunction and is associated with cardiovascular diseases in patients with chronic kidney diseases (CKD). However, the underlying mechanism of high Pi-induced endothelia cell apoptosis remains unclear.

Methods

Human umbilical vein endothelial cells (HUVECs) were treated with normal Pi (1.0 mM) and high Pi (3.0 mM), and then cell apoptosis, abnormal gene expression and potential signaling pathway involvement in simulated hyperphosphatemia were examined using flow cytometry, quantitative PCR (qPCR) and western blot analysis. A two-step 5/6 nephrectomy was carried out to induce CKD and biochemical measurements were taken.

Results

The rat model

SUBMITTER: Wang S 

PROVIDER: S-EPMC7307556 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature