Proteomic Toolbox To Standardize the Separation of Extracellular Vesicles and Lipoprotein Particles.

ABSTRACT: Circulating in blood, extracellular vesicles (EVs) and lipoprotein particles (LPs) have diagnostic and prognostic value. To unambiguously define their functions, separation protocols need to be developed. However, because of their similar size and density, traditional approaches to separate EVs and LPs often fail to provide the required resolution. Further development and standardization of affinity-based protocols is necessary, and a quantitative method is needed to assess the efficiency of LP depletion from EV samples. In the present study, we propose the simultaneous quantification of three groups of proteins by mass spectrometry as a toolbox to evaluate prospective separation protocols. We generated ¹⁵N-labeled internal standards for quantification of (i) EV-specific protein

SUBMITTER: Wang T 

PROVIDER: S-EPMC7307575 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature