Identification of Adenosine-to-Inosine RNA Editing with Acrylonitrile Reagents.

ABSTRACT: New chemical probes have been designed to facilitate the identification of adenosine-to-inosine (A-to-I) edited RNAs. These reagents combine a conjugate acceptor for selective inosine covalent modification with functional groups for bioorthogonal biotinylation. The resulting biotinylated RNA was enriched and verified with RT-qPCR. This powerful chemical approach provides new opportunities to identify and quantify A-to-I editing sites.

SUBMITTER: Li Y 

PROVIDER: S-EPMC7308179 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature