Comprehensive Immunoprofiling of Pediatric Zika Reveals Key Role for Monocytes in the Acute Phase and No Effect of Prior Dengue Virus Infection.

ABSTRACT: Zika virus (ZIKV) is an emerging, mosquito-borne flavivirus responsible for recent epidemics across the Americas, and it is closely related to dengue virus (DENV). Here, we study samples from 46 DENV-naive and 43 DENV-immune patients with RT-PCR-confirmed ZIKV infection at early-acute, late-acute, and convalescent time points from our pediatric cohort study in Nicaragua. We analyze the samples via RNA sequencing (RNA-seq), CyTOF, and multiplex cytokine/chemokine Luminex to generate a comprehensive, innate immune profile during ZIKV infection. Immunophenotyping and analysis of cytokines/chemokines reveal that CD14⁺ monocytes play a key role during ZIKV infection. Further, we identify CD169 (Siglec-1) on CD14⁺ monocytes as a potential biomarker of acute ZIKV infection.

SUBMITTER: Michlmayr D 

PROVIDER: S-EPMC7308490 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature