Transcriptomic analysis reveals diverse gene expression changes in airway macrophages during experimental allergic airway disease.

ABSTRACT: Background: Airway macrophages (AMs) are the most abundant leukocytes in the healthy airway lumen and have a highly specialised but plastic phenotype that is governed by signals in the local microenvironment. AMs are thought to maintain immunological homeostasis in the steady state, but have also been implicated in the pathogenesis of allergic airway disease (AAD). In this study, we aimed to better understand these potentially contrasting AM functions using transcriptomic analysis. Methods: Bulk RNA sequencing was performed on AMs (CD11c ⁺ Siglec F ⁺ CD64 ⁺ CD45 ⁺ SSC ^hi) flow cytometry sorted from C57BL/6 mice during experimental AAD driven by repeated house dust mite inhalation (AMs ^HDM), compared to control

SUBMITTER: Branchett WJ 

PROVIDER: S-EPMC7309452 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature