Fam70A binds Wnt5a to regulate meiosis and quality of mouse oocytes.

ABSTRACT: OBJECTIVES:Little is known about the roles of integral membrane proteins beyond channels, carriers or receptors in meiotic oocytes. The transmembrane protein Fam70A was previously identified as a likely "female fertility factor" in Fox3a-knockout mouse ovaries where almost all follicles underwent synchronous activation and the mice became infertile very early. However, whether Fam70A functions in oocyte meiosis remains unknown. Therefore, the present study aimed to address this question. MATERIALS AND METHODS:Co-immunoprecipitation, immunogold labelling-electron microscopy, co-localization and yeast two-hybrid assays were used to verify the interaction. Antibody or small interfering RNA transfection was used to deplete the proteins. Immunofluorescence, immunohistochemistry and live tracker

SUBMITTER: Zhang NN 

PROVIDER: S-EPMC7309945 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature