An approach using Caenorhabditis elegans screening novel targets to suppress tumour cell proliferation.

ABSTRACT:

Objectives

Tumour cell proliferation requires high metabolism to meet the bioenergetics and biosynthetic needs. Dauer in Caenorhabditis elegans is characterized by lower metabolism, and we established an approach with C elegans to find potential tumour therapy targets.

Materials and methods

RNAi screening was used to find dauer-related genes, and these genes were further analysed in glp-1(-) mutants for tumour-suppressing testing. The identified tumour-related genes were verified in clinical tumour tissues.

Results

The lifespan of glp-1(-) mutants was found to be extended by classical dauer formation signalling. Then, 61 of 287 kinase-coding genes in Caenorhabditis elegans were identified as dauer-related genes, of which 27 were found to be homologous to human oncogen

SUBMITTER: Mao YQ 

PROVIDER: S-EPMC7309951 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature