Replication origin location might contribute to genetic variability in Trypanosoma cruzi.

ABSTRACT:

Background

DNA replication in trypanosomatids operates in a uniquely challenging environment, since most of their genomes are constitutively transcribed. Trypanosoma cruzi, the etiological agent of Chagas disease, presents high variability in both chromosomes size and copy number among strains, though the underlying mechanisms are unknown.

Results

Here we have mapped sites of DNA replication initiation across the T. cruzi genome using Marker Frequency Analysis, which has previously only been deployed in two related trypanosomatids. The putative origins identified in T. cruzi show a notable enrichment of GC content, a preferential position at subtelomeric regions, coinciding with genes transcribed towards the telomeres, and a pronounced enrichment within coding DNA sequences,

SUBMITTER: de Araujo CB 

PROVIDER: S-EPMC7310030 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature