What is the response profile of deciduous pulp fibroblasts stimulated with E. coli LPS and E. faecalis LTA?

ABSTRACT:

Background

Oral fibroblast immunological responses to bacterial stimuli are well known. However, there are few studies about pulp fibroblasts from deciduous teeth (HDPF) responses, which are important for the treatment of pulp infections in children. The aim of this study was to evaluate expression and production of inflammatory cytokines and chemokines by HDPF when challenged with bacterial antigens normally present in advanced caries lesions.

Methods

Triplicate HDPF from 4 children (n = 4; 2 boys and 2 girls) were cultured by explant technique and challenged or not with Escherichia coli lipopolysaccharide/1 μg/mL (EcLPS) or Enterococcus faecalis lipoteichoic acid/1 μg/mL (EfLTA) for 6 and 24 h. Most of published studies employed immortalized cells, i.e., without checking p

SUBMITTER: Colombini-Ishikiriama BL 

PROVIDER: S-EPMC7310245 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature