New generation breast cancer cell lines developed from patient-derived xenografts.

ABSTRACT:

Background

Breast cancer is a highly heterogeneous disease characterized by multiple histologic and molecular subtypes. While a myriad of breast cancer cell lines have been developed over the past 60 years, estrogen receptor alpha (ER)+ disease and some mutations associated with this subtype remain underrepresented. Here we describe six breast cancer cell lines derived from patient-derived xenografts (PDX) and their general characteristics.

Methods

Established breast cancer PDX were processed into cell suspensions and placed into standard 2D cell culture; six emerged into long-term passageable cell lines. Cell lines were assessed for protein expression of common luminal, basal, and mesenchymal markers, growth assessed in response to estrogens and endocrine therapies, and RNA

SUBMITTER: Finlay-Schultz J 

PROVIDER: S-EPMC7310532 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature