Phosphoproteomics of short-term hedgehog signaling in human medulloblastoma cells.

ABSTRACT:

Background

Aberrant hedgehog (HH) signaling is implicated in the development of various cancer entities such as medulloblastoma. Activation of GLI transcription factors was revealed as the driving force upon pathway activation. Increased phosphorylation of essential effectors such as Smoothened (SMO) and GLI proteins by kinases including Protein Kinase A, Casein Kinase 1, and Glycogen Synthase Kinase 3 β controls effector activity, stability and processing. However, a deeper and more comprehensive understanding of phosphorylation in the signal transduction remains unclear, particularly during early response processes involved in SMO activation and preceding GLI target gene regulation.

Methods

We applied temporal quantitative phosphoproteomics to reveal phosphorylation dynami

SUBMITTER: Scheidt T 

PROVIDER: S-EPMC7310537 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature