Ginkgolide B inhibits lung cancer cells promotion via beclin-1-dependent autophagy.

ABSTRACT:

Background

Ginkgolide B (GKB) is a major active component of the extracts of Ginkgo biloba leaves, and it has been used as an anti-cancer agent. However, it is unknown whether GKB has the therapeutic effects on lung cancer. Here, we studied the effects of GKB on lung cancer cells.

Methods

The effects of GKB on lung cancer cell proliferation and invasion were analyzed by cell counting kit (CCK-8) and cell invasion assays, respectively. Apoptosis was detected by flow cytometry. Western blot analysis was used to confirm the expression of autophagy-associated proteins in GKB-treated cells. Immunofluorescence analysis was used to analyze the level of light chain 3B (LC3B).

Results

Treatment with GKB time-dependently inhibited the proliferation and decreased the invasive ca

SUBMITTER: Wang X 

PROVIDER: S-EPMC7310550 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature