Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis.

ABSTRACT: Coronavirus disease 2019 ( COVID-19 ) is a global health crisis caused by the novel severe acute respiratory syndrome coronavirus 2 ( SARS-CoV-2 ), and there is a critical need to produce large quantities of high-quality SARS-CoV-2 Spike ( S ) protein for use in both clinical and basic science settings. To address this need, we have evaluated the expression and purification of two previously reported S protein constructs in Expi293F ^™ and ExpiCHO-S ^™ cells, two different cell lines selected for increased expression of secreted glycoproteins. We show that ExpiCHO-S ^™ cells produce enhanced yields of both SARS-CoV-2 S proteins. Biochemical, biophysical, and structural ( cryo-EM ) characterization of the SARS-CoV-2 S proteins produc

SUBMITTER: Herrera NG 

PROVIDER: S-EPMC7310628 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature