Cryo-EM structure of the SARS-CoV-2 3a ion channel in lipid nanodiscs.

ABSTRACT: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus that causes the coronavirus disease 2019 (COVID-19). SARS-CoV-2 encodes three putative ion channels: E, 8a, and 3a^1,2. 3a is expressed in SARS patient tissue and anti-3a antibodies are observed in patient plasma^3-6. 3a has been implicated in viral release⁷, inhibition of autophagy⁸, inflammasome activation⁹, and cell death^10,11 and its deletion reduces viral titer and morbidity in mice¹, raising the possibility that 3a could be an effective vaccine or therapeutic target^3,12. Here, we present the first cryo-EM structures of SARS-CoV-2 3a to 2.1 Å resolution and demonstrate 3a forms an ion channel in reconstituted liposomes. The str

SUBMITTER: Kern DM 

PROVIDER: S-EPMC7310636 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature