A multicolor multiplex lateral flow assay for high-sensitivity analyte detection using persistent luminescent nanophosphors.

ABSTRACT: Incorporating two persistent luminescent nanophosphors (PLNPs), green-emitting SrAl₂O₄:Eu²⁺,Dy³⁺ (SAO) and blue-emitting (Sr_0.625Ba_0.375)₂MgSi₂O₇:Eu²⁺,Dy³⁺ (SBMSO), in a single lateral flow assay (LFA) establishes a luminescence-based, multiplex point-of-need test capable of simultaneously detecting two different analytes in a single sample. The advantages of this system are the high sensitivity and photostability of PLNPs, while only requiring access to minimal hardware and a smartphone for signal detection. The PLNPs were obtained by first wet milling bulk synthesized phosphor powders, followed by fractionation using differential centrifugal sedimentation to obtain mon

SUBMITTER: Danthanarayana AN 

PROVIDER: S-EPMC7310964 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature