Increased mAb production in amplified CHO cell lines is associated with increased interaction of CREB1 with transgene promoter.

ABSTRACT: Most therapeutic monoclonal antibodies in biopharmaceutical processes are produced in Chinese hamster ovary (CHO) cells. Technological advances have rendered the selection procedure for higher producers a robust protocol. However, information on molecular mechanisms that impart the property of hyper-productivity in the final selected clones is currently lacking. In this study, an IgG-producing industrial cell line and its methotrexate (MTX)-amplified progeny cell line were analyzed using transcriptomic, proteomic, phosphoproteomic, and chromatin immunoprecipitation (ChIP) techniques. Computational prediction of transcription factor binding to the transgene cytomegalovirus (CMV) promoter by the Transcription Element Search System and upstream regulator analysis of the differential transcrip

SUBMITTER: Dahodwala H 

PROVIDER: S-EPMC7311070 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature