Establishment and characterization of an embryonic pericyte cell line.

ABSTRACT:

Objective

Pericytes are specialized perivascular cells embedded within the basement membrane. These cells envelope the abluminal surface of endothelial cells and promote microvessel homeostasis. Recent discoveries of unique pericyte functions, particularly in neural tissues, underscore the need for overcoming existing challenges in establishing a functionally validated pericyte cell line. Here, we present methodologies for addressing these challenges as well as an embryonic pericyte cell line for use with in vitro and ex vivo experimental models.

Methods

We isolated an enriched population of NG2:DsRed+ pericytes from E12.5 mice. This pericyte cell line was compared to MEFs with respect to gene expression, cell morphology and migration, and engagement with endothelial cells d

SUBMITTER: Zhao H 

PROVIDER: S-EPMC7311072 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature