Injectable RANKL sustained release formulations to accelerate orthodontic tooth movement.

ABSTRACT:

Background

Accelerating orthodontic tooth movement (OTM) through biologically effective methods, such as increasing osteoclast-mediated alveolar resorption, could effectively shorten treatment time.

Objective

To evaluate an injectable formulation containing receptor activator of nuclear factor kappa-B ligand (RANKL) on the OTM.

Materials and methods

We fabricated a RANKL formulation from 100 µl of 100 µg/ml RANKL adsorbed on 10 mg of poly(lactic acid-co-glycolic acid) microspheres embedded in a 10 wt% aqueous hydroxyethyl cellulose carrier gel. We characterized these formulations for the rate of RANKL release, and then tested for bioactivity using in vitro cell culture. In vivo OTM studies were conducted using 15 week old male Wistar rats for 14 days. We injected the

SUBMITTER: Chang JH 

PROVIDER: S-EPMC7311078 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature