Advantages and Limits of Metagenomic Assembly and Binning of a Giant Virus.

ABSTRACT: Giant viruses have large genomes, often within the size range of cellular organisms. This distinguishes them from most other viruses and demands additional effort for the successful recovery of their genomes from environmental sequence data. Here, we tested the performance of genome-resolved metagenomics on a recently isolated giant virus, Fadolivirus, by spiking it into an environmental sample from which two other giant viruses were isolated. At high spike-in levels, metagenome assembly and binning led to the successful genomic recovery of Fadolivirus from the sample. A complementary survey of the major capsid protein indicated the presence of other giant viruses in the sample matrix but did not detect the two isolated from this sample. Our results indicate that genome-resolved metagenomi

SUBMITTER: Schulz F 

PROVIDER: S-EPMC7311315 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature