Bioinformatics analysis and characterization of a secretory cystatin from Thelohanellus kitauei.

ABSTRACT: Thelohanellus kitauei, is a member of obligate parasitic myxozoans, which causes intestinal giant-cystic disease of common carp (Cyprinus carpio) and has resulted in significant economic losses in carp farms. Cystatin secreted by parasites can regulate the immune response of host to facilitate parasite's survival. In this study, the secretory TK-cystatin gene, encoding a protein of 120 amino acid residues (13.65 kDa), was cloned from T. kitauei genome. Phylogenetic analysis showed that TK-cystatin gene is closely related to the cystatin-A from Hydra vulgaris. Multiple sequence alignment revealed that TK-cystatin had three conserved motifs: N-terminal G¹⁹G²⁰, Q⁷³VVAG⁷⁷, and C-terminal L¹⁰²P¹⁰³. Molecular docking between TK-cy

SUBMITTER: Zhang F 

PROVIDER: S-EPMC7311561 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature