Isolation and Genotypic Characterization of Toxoplasma gondii Based on GRA6 Gene from Environmental Soil Samples in Mazandaran Province, North of Iran.

ABSTRACT:

Background

Soil is one of the environmental sources of Toxoplasma gondii oocysts. The other hand, genotype of the parasite is one of the important factors for its pathogenicity. Due to the importance of toxoplasmosis on public health, this study aimed to isolation and genotyping of T. gondii in environmental soil samples of Mazandaran Province, north of Iran.

Methods

Overall, 192 soil samples were collected from different areas in Mazandaran Province from Apr to Sep 2014. The flotation method was used for recovering oocysts. Then, soil samples were investigated for DNA detection of T. gondii using nested PCR of RE gene, genotyping with Semi-nested PCR of GRA6 gene and restriction fragment length polymorphism (RFLP) analysis. Results were analyzed using C

SUBMITTER: Haghparast-Kenari B 

PROVIDER: S-EPMC7311813 | biostudies-literature | 2020 Apr-Jun

REPOSITORIES: biostudies-literature