Molecular detection and genetic variability of Ehrlichia canis in pet dogs in Xinjiang, China.

ABSTRACT:

Background and aim

As a tick-borne zoonotic pathogen, Ehrlichia canis has already posed a threat to public health and safety. This study aimed to clarify the prevalence and molecular characteristics of E. canis in pet dogs in Xinjiang, China.

Materials and methods

A total of 297 blood samples of pet dogs and 709 skin ticks (Rhipicephalus sanguineus sensu lato) were subjected to molecular detection using PCR for E. canis 16S rRNA gene, and then, positive samples were amplified, sequenced, and phylogenetically analyzed for E. canis gp36 gene.

Results

The PCR detection showed that the positive rate of PCR was 12.12% (36/297) in blood samples and 15.23% (108/709) in tick samples, respectively. Based on the phylogenetic anal

SUBMITTER: Mengfan Q 

PROVIDER: S-EPMC7311875 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature