Source of dopamine in gastric juice and luminal dopamine-induced duodenal bicarbonate secretion via apical dopamine D₂ receptors.

ABSTRACT:

Background and purpose

Dopamine protects the duodenal mucosa. Here we have investigated the source of dopamine in gastric juice and the mechanism underlying the effects of luminal dopamine on duodenal bicarbonate secretion (DBS) in rodents.

Experimental approach

Immunofluorescence, UPLC-MS/MS, gastric incubation and perfusion were used to detect gastric-derived dopamine. Immunofluorescence and RT-PCR were used to examine the expression of dopamine receptors in the duodenal mucosa. Real-time pH titration and pH_i measurement were performed to investigate DBS.

Key results

H⁺ -K⁺ -ATPase was co-localized with tyrosine hydroxylase and dopamine transporters in gastric parietal cells. Dopamine was increased in in vivo gastric perfusate after

SUBMITTER: Feng XY 

PROVIDER: S-EPMC7312307 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature