Transcriptional Activity and Stability of CD39+CD103+CD8+ T Cells in Human High-Grade Endometrial Cancer.

ABSTRACT: Tumor-infiltrating CD8+ T cells (TIL) are of the utmost importance in anti-tumor immunity. CD103 defines tumor-resident memory T cells (T_RM cells) associated with improved survival and response to immune checkpoint blockade (ICB) across human tumors. Co-expression of CD39 and CD103 marks tumor-specific T_RM with enhanced cytolytic potential, suggesting that CD39+CD103+ T_RM could be a suitable biomarker for immunotherapy. However, little is known about the transcriptional activity of T_RM cells in situ. We analyzed CD39+CD103+ T_RM cells sorted from human high-grade endometrial cancers (n = 3) using mRNA sequencing. Cells remained untreated or were incubated with PMA/ionomycin (activation), actinomycin D (a platinum-like chemotherap

SUBMITTER: Workel HH 

PROVIDER: S-EPMC7312498 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature