Concordance between Thioacetamide-Induced Liver Injury in Rat and Human In Vitro Gene Expression Data.

ABSTRACT: The immense resources required and the ethical concerns for animal-based toxicological studies have driven the development of in vitro and in silico approaches. Recently, we validated our approach in which the expression of a set of genes is uniquely associated with an organ-injury phenotype (injury module), by using thioacetamide, a known liver toxicant. Here, we sought to explore whether RNA-seq data obtained from human cells (in vitro) treated with thioacetamide-S-oxide (a toxic intermediate metabolite) would correlate across species with the injury responses found in rat cells (in vitro) after exposure to this metabolite as well as in rats exposed to thioacetamide (in vivo). We treated two human cell types with thioacetamide-S-oxide (primary hepatocytes with 0 (vehicle), 0.125 (low dos

SUBMITTER: Schyman P 

PROVIDER: S-EPMC7312807 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature