Molecular Cytogenetic Identification of Wheat-Aegilops Biuncialis 5M^b Disomic Addition Line with Tenacious and Black Glumes.

ABSTRACT: Production of wheat-alien disomic addition lines is of great value to the exploitation and utilization of elite genes originated from related species to wheat. In this study, a novel wheat-Aegilops biuncialis 5M^b disomic addition line WA317 was characterized by in situ hybridization (ISH) and specific-locus amplified fragment sequencing (SLAF-seq) markers. Compared to its parent Chinese Spring (CS), the glumes of WA317 had black color and were difficult to remove after harvesting, suggesting chromosome 5M^b carried gene(s) related to glume development and Triticeae domestication process. A total of 242 Ae. biuncialis SLAF-based markers (298 amplified patterns) were developed and further divided into four categories by Ae. biuncialis Y17,

SUBMITTER: Song L 

PROVIDER: S-EPMC7312955 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature