A video protocol for rapid dissection of mouse dorsal root ganglia from defined spinal levels.

ABSTRACT:

Objective

Dorsal root ganglia (DRG) are heterogeneous assemblies of assorted sensory neuron cell bodies found in bilateral pairs at every level of the spinal column. Pseudounipolar afferent neurons convert external stimuli from the environment into electrical signals that are retrogradely transmitted to the spinal cord dorsal horn. To do this, they extend single axons from their DRG-resident somas that then bifurcate and project both centrally and distally. DRG can be dissected from mice at embryonic stages and any age post-natally, and have been extensively used to study sensory neuron development and function, response to injury, and pathological processes in acquired and genetic diseases. We have previously published a step-by-step dissection method for the rapid isolation of po

SUBMITTER: Sleigh JN 

PROVIDER: S-EPMC7313212 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature