Sequential deletion of CD63 identifies topologically distinct scaffolds for surface engineering of exosomes in living human cells.

ABSTRACT: Exosomes are cell-derived extracellular vesicles that have great potential in the field of nano-medicine. However, a fundamental challenge in the engineering of exosomes is the design of biocompatible molecular scaffolds on their surface to enable cell targeting and therapeutic functions. CD63 is a hallmark protein of natural exosomes that is highly enriched on the external surface of the membrane. We have previously described engineering of CD63 for use as a molecular scaffold in order to introduce cell-targeting features to the exosome surface. Despite this initial success, the restrictive M-shaped topology of full-length CD63 may hinder specific applications that require N- or C-terminal display of cell-targeting moieties on the outer surface of the exosome. In this study, we describe n

SUBMITTER: Curley N 

PROVIDER: S-EPMC7313400 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature