Room-temperature-storable PCR mixes for SARS-CoV-2 detection.

ABSTRACT:

Objectives

A novel coronavirus (severe acute respiratory syndrome coronavirus 2, SARS-CoV-2) emerged in late 2019, causing an outbreak of pneumonia [coronavirus disease 2019 (COVID-19)] globally. Although the use of ready-made reaction mixes can enable more rapid PCR-based diagnosis of COVID-19, the need to transport and store these mixes at low temperatures presents challenges to already overburdened logistics networks.

Methods

Here, we present an optimized freeze-drying procedure that allows SARS-CoV-2 PCR mixes to be transported and stored at ambient temperatures, without loss of activity. Additive-supplemented PCR mixes were freeze-dried. The residual moisture of the freeze-dried PCR mixes was measured by Karl-Fischer titration.

Results

We found that the freeze-dr

SUBMITTER: Xu J 

PROVIDER: S-EPMC7313492 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature