Regenerative potential of prostate luminal cells revealed by single-cell analysis.

ABSTRACT: Androgen deprivation is the cornerstone of prostate cancer treatment. It results in involution of the normal gland to ~90% of its original size because of the loss of luminal cells. The prostate regenerates when androgen is restored, a process postulated to involve stem cells. Using single-cell RNA sequencing, we identified a rare luminal population in the mouse prostate that expresses stemlike genes (Sca1 ⁺ and Psca ⁺) and a large population of differentiated cells (Nkx3.1 ⁺, Pbsn ⁺). In organoids and in mice, both populations contribute equally to prostate regeneration, partly through androgen-driven expression of growth factors (Nrg2, Rspo3) by mesenchymal cells acting in a paracrine fashion on luminal cells. Analys

SUBMITTER: Karthaus WR 

PROVIDER: S-EPMC7313621 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature