Toxin transcripts in Crotalus atrox venom and in silico structures of toxins.

ABSTRACT: The western diamondback rattlesnake (Crotalus atrox) is a common and widespread North American pit viper species, and its venom possesses medical applications. In this research, we identified 14 of the most common transcripts encoding 11 major venom toxins including transcripts for a three-finger toxin (3FTx) from the crude venom of C. atrox. In silico three-dimensional (3D) structures of 9 venom toxins were predicted by using deduced toxin amino acid sequences and a computer programme-MODELLER. The accuracy of all predicted toxin structures was evaluated by five stereochemical structure parameters including discrete optimised protein energy (DOPE) score, root mean square deviation (RMSD), Z-score, overall quality factor (ERRAT), and φ/ψ dihedral angle distribution of toxin b

SUBMITTER: Jia Y 

PROVIDER: S-EPMC7314381 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature