Applications of single-cell sequencing for the field of otolaryngology: A contemporary review.

ABSTRACT:

Objectives

Single-cell RNA sequencing (scRNA-Seq) is a new technique used to interrogate the transcriptome of individual cells within native tissues that have already resulted in key discoveries in auditory basic science research. Rapid advances in scRNA-Seq make it likely that it will soon be translated into clinical medicine. The goal of this review is to inspire the use of scRNA-Seq in otolaryngology by giving examples of how it can be applied to patient samples and how this information can be used clinically.

Methods

Studies were selected based on the scientific quality and relevance to scRNA-Seq. In addition to mouse auditory system (inner ear including hair cells and supporting cells, spiral ganglion neurons, and inner ear organoids), recent studies using human primary

SUBMITTER: Pyle MP 

PROVIDER: S-EPMC7314468 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature