Cell atlas of the foetal human heart and implications for autoimmune-mediated congenital heart block.

ABSTRACT:

Aims

Investigating human heart development and applying this to deviations resulting in disease is incomplete without molecular characterization of the cell types required for normal functioning. We investigated foetal human heart single-cell transcriptomes from mid-gestational healthy and anti-SSA/Ro associated congenital heart block (CHB) samples.

Methods and results

Three healthy foetal human hearts (19th to 22nd week of gestation) and one foetal heart affected by autoimmune-associated CHB (21st week of gestation) were subjected to enzymatic dissociation using the Langendorff preparation to obtain single-cell suspensions followed by 10× Genomics- and Illumina-based single-cell RNA-sequencing (scRNA-seq). In addition to the myocytes, fibroblasts, immune cells, and other mi

SUBMITTER: Suryawanshi H 

PROVIDER: S-EPMC7314636 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature