Transcriptome Analysis of Zygotic Induction During Conjugative Transfer of Plasmid RP4.

ABSTRACT: Conjugative transfer of bacterial plasmid is one of the major mechanisms of horizontal gene transfer, which is mediated by direct contact between donor and recipient cells. Gene expression of a conjugative plasmid is tightly regulated mostly by plasmid-encoded transcriptional regulators, but it remains obscure how differently plasmid genes are expressed in each cell during the conjugation event. Here, we report a comprehensive analysis of gene expression during conjugative transfer of plasmid RP4, which is transferred between isogenic strains of Pseudomonas putida KT2440 at very high frequency. To discriminate the expression changes in the donor and recipient cells, we took advantage of conjugation in the presence of rifampicin (Rif). Within 10 min of mating, we successfully detecte

SUBMITTER: Miyakoshi M 

PROVIDER: S-EPMC7314908 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature