Discovery of CD80 and CD86 as recent activation markers on regulatory T cells by protein-RNA single-cell analysis.

ABSTRACT:

Background

Traditionally, the transcriptomic and proteomic characterisation of CD4⁺ T cells at the single-cell level has been performed by two largely exclusive types of technologies: single-cell RNA sequencing (scRNA-seq) and antibody-based cytometry. Here, we present a multi-omics approach allowing the simultaneous targeted quantification of mRNA and protein expression in single cells and investigate its performance to dissect the heterogeneity of human immune cell populations.

Methods

We have quantified the single-cell expression of 397 genes at the mRNA level and up to 68 proteins using oligo-conjugated antibodies (AbSeq) in 43,656 primary CD4⁺ T cells isolated from the blood and 31,907 CD45⁺ cells isolated from the blood and matched duod

SUBMITTER: Trzupek D 

PROVIDER: S-EPMC7315544 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature