Co-localization and confinement of ecto-nucleotidases modulate extracellular adenosine nucleotide distributions.

ABSTRACT: Nucleotides comprise small molecules that perform critical signaling roles in biological systems. Adenosine-based nucleotides, including adenosine tri-, di-, and mono-phosphate, are controlled through their rapid degradation by diphosphohydrolases and ecto-nucleotidases (NDAs). The interplay between nucleotide signaling and degradation is especially important in synapses formed between cells, which create signaling 'nanodomains'. Within these 'nanodomains', charged nucleotides interact with densely-packed membranes and biomolecules. While the contributions of electrostatic and steric interactions within such nanodomains are known to shape diffusion-limited reaction rates, less is understood about how these factors control the kinetics of nucleotidase activity. To quantify these factors, we

SUBMITTER: Rahmaninejad H 

PROVIDER: S-EPMC7316229 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature