Photostable and Proteolysis-Resistant Forster Resonance Energy Transfer-Based Calcium Biosensor.

ABSTRACT: Molecular sensors from protein engineering offer new methods to sensitively bind to and detect target analytes for a wide range of applications. For example, these sensors can be integrated into probes for implantation, and then yield new and valuable physiological information. Here, a new Förster resonance energy transfer (FRET)-based sensor is integrated with an optical fiber to yield a device measuring free Ca²⁺. This membrane encapsulated optical fiber (MEOF) device is composed of a sensor matrix that fills poly(tetrafluoroethylene) (PTFE) with an engineered troponin C (TnC) protein fused to a pair of FRET fluorophores. The FRET efficiency is modulated upon Ca²⁺ ion binding. The probe further comprises a second, size-excluding filter membrane that is synthesized b

SUBMITTER: Nguyen D 

PROVIDER: S-EPMC7316412 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature