Generation and characterization of a cell line from an intraductal tubulopapillary neoplasm of the pancreas.

ABSTRACT: Intraductal tubulopapillary neoplasm (ITPN) is a distinct precancerous lesion in the pancreas with unique clinical and molecular features. Although in vitro studies in two-dimensional culture have led to numerous important insights in pancreatic cancer, such models are currently lacking for precancerous lesions. In this study, we report the generation and characterization of a cell line from a human pancreatic ITPN. Neoplastic cells were initially cultured in a three-dimensional organoid system, followed by transfer to two-dimensional culture. RNA sequencing revealed a gene expression profile consistent with pancreatic ductal origin, and whole genome sequencing identified many somatic mutations (including in genes involved in DNA repair and Wnt signaling) and structural rearrangements. In

SUBMITTER: Felsenstein M 

PROVIDER: S-EPMC7316603 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature