Differential DNA methylation patterns in human Schlemm's canal endothelial cells with glaucoma.

ABSTRACT:

Purpose

Schlemm's canal (SC) endothelial cells derived from donors with or without glaucoma showed different mechanical properties and gene expression. As an important contributor to the regulation of intraocular pressure (IOP) and pathogenesis of primary open-angle glaucoma (POAG), the heritable key epigenetic changes, methylation may play an important role in the physiologic function of SC cells. This study aims to identify differentially methylated CpG sites (DMSs) in primary cultures of human SC cells with or without glaucoma.

Methods

We examined the methylation pattern of seven strains of primary human cells (two glaucoma and five normal SC cell samples), which were isolated and characterized using established protocols. DNA methylation was profiled using Illumina Human

SUBMITTER: Cai J 

PROVIDER: S-EPMC7316632 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature