Specific, sensitive and rapid authentication of donkey-hide gelatine (Colla corii asini) in processed food using an isothermal nucleic acid amplification assay.

ABSTRACT: Donkey-hide gelatine (DHG) is a well-known, animal-derived traditional Chinese medicine material called Colla corii asini (known in Chinese as "E'jiao"). Because DHG is claimed to have properties that are beneficial to health, its consumption has increased, but its production has decreased. Thus, the incidence of DHG adulteration has become increasingly serious. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for the authentication of DHG. Identification of donkey DNA from DHG was performed specifically and rapidly within one hour by LAMP primers. Moreover, the sensitivity of LAMP in authenticating DHG was 10^-3 ng, which revealed a 10⁵-fold higher sensitivity than that of conventional PCR. The relative detection limit was 0.1%

SUBMITTER: Sheu SC 

PROVIDER: S-EPMC7316948 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature