Next-Generation Manufacturing Protocols Enriching T_SCM CAR T Cells Can Overcome Disease-Specific T Cell Defects in Cancer Patients.

ABSTRACT: Chimeric antigen receptor (CAR) T cell expansion and persistence emerged as key efficacy determinants in cancer patients. These features are typical of early-memory T cells, which can be enriched with specific manufacturing procedures, providing signal one and signal two in the proper steric conformation and in the presence of homeostatic cytokines. In this project, we exploited our expertise with paramagnetic beads and IL-7/IL-15 to develop an optimized protocol for CAR T cell production based on reagents, including a polymeric nanomatrix, which are compatible with automated manufacturing via the CliniMACS Prodigy. We found that both procedures generate similar CAR T cell products, highly enriched of stem cell memory T cells (T_SCM) and equally effective in counteracting

SUBMITTER: Arcangeli S 

PROVIDER: S-EPMC7317024 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature